Journal: Cells

Article Title: Optical Microscopy and the Extracellular Matrix Structure: A Review

doi: 10.3390/cells10071760

Figure Lengend Snippet: Schematic overview of extracellular matrix and its major components. Although the ECM composition varies depending on the tissue, the matrix is mainly composed of a variety of fibrous proteins (collagen, elastin, fibronectin, and laminin) and polysaccharides that are secreted locally and assembled into an organized meshwork in close association with the surface of the cell that produced them.

Article Snippet: TIRFM , Good SNR due to low penetration depth of the evanescent field Reduced photobleaching , Sectioning is confined to a single-plane immediately adjacent to the cover glass or slide , Fibronectin Crosslinking of ECM fibrillar proteins Cell adhesion points Endo-exocytosis Cytoskeleton dynamics.

Techniques: Produced

Journal: Cells

Article Title: Optical Microscopy and the Extracellular Matrix Structure: A Review

doi: 10.3390/cells10071760

Figure Lengend Snippet: The most commonly used linear fluorescence-based super-resolution microscopy modalities to assess ECM components.

Article Snippet: TIRFM , Good SNR due to low penetration depth of the evanescent field Reduced photobleaching , Sectioning is confined to a single-plane immediately adjacent to the cover glass or slide , Fibronectin Crosslinking of ECM fibrillar proteins Cell adhesion points Endo-exocytosis Cytoskeleton dynamics.

Techniques: Fluorescence, Super-Resolution Microscopy, Imaging, In Vivo, Control, Modification, Microscopy

Journal: Cells

Article Title: Optical Microscopy and the Extracellular Matrix Structure: A Review

doi: 10.3390/cells10071760

Figure Lengend Snippet: Representative images of different ECM components acquired by ( A ) WFFM, ( B ) LSCM, ( C ) STED/GSD, ( D , E ) PALM, and ( F ) STORM. ( A ) Shows a widefield fluorescence image of fibroblasts and matrix followed by images of Alexa-647-labeled fibronectin and antibody-stained collagen I alone. Scale bar, 100 μm . ( B ) Image obtained by confocal microscopy of fibronectin deposition and localization in 3D co-cultures of human breast cancer cells and human dermal fibroblasts. Cultures were labelled to visualize actin (red), fibronectin (green), and cell nuclei (blue). Scale bar, 50 μm . ( C ) Higher magnification SUSHI (super-resolution shadow imaging) images of cell bodies and neuropil in CA1 area. Scale bar in top, 4 mm; middle, 5 mm; bottom, 2 mm . ( D ) Schematic of sample setup for iPALM imaging of migratory Jurkat T-lymphocytes adhered to ICAM-1 or fibronectin-coated lower coverslips, with gold nanorod fiducial markers (orange spheres) . ( E ) Representative iPALM renderings of Jurkat cells expressing mEOS3.2-LFA-1 fusion, mEOS3.2-CAAX, or LifeAct-mEos3.2. Scale bars, 5 mm . ( F ) STORM images of mineralized collagen fibrils at an early stage (top). Representative Z-dimension slices of the STORM images (bottom). Amorphous calcium phosphate (ACP) preferentially aggregates around purple in the intrafibrillar compartments, which represent chondroitin sulfate (CS) in CS-collagen fibril. Scale bar: 500 nm . Images reproduced with permission from [ , , , , ].

Article Snippet: TIRFM , Good SNR due to low penetration depth of the evanescent field Reduced photobleaching , Sectioning is confined to a single-plane immediately adjacent to the cover glass or slide , Fibronectin Crosslinking of ECM fibrillar proteins Cell adhesion points Endo-exocytosis Cytoskeleton dynamics.

Techniques: Fluorescence, Labeling, Staining, Confocal Microscopy, Imaging, Expressing

Journal: Cells

Article Title: Optical Microscopy and the Extracellular Matrix Structure: A Review

doi: 10.3390/cells10071760

Figure Lengend Snippet: Representative examples of applications of different microscopy modalities to assess ECM main components.

Article Snippet: TIRFM , Good SNR due to low penetration depth of the evanescent field Reduced photobleaching , Sectioning is confined to a single-plane immediately adjacent to the cover glass or slide , Fibronectin Crosslinking of ECM fibrillar proteins Cell adhesion points Endo-exocytosis Cytoskeleton dynamics.

Techniques: Microscopy, Imaging